Sample information
Picture	Photos by: Nathan N.
Location
Collection date	08/10/2023
Captive / Cultivated?	Captive / Cultivated
Group	Galileo Academy of Science and Technology
Observations	It had a proboscus, slender legs, wings
Putative identification	Arthropoda Hexapoda Insecta Diptera Culicidae Culex Culex pipiens
Methods
Extraction kit	BABEC
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Did not produce 2nd green band of dna
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Culex pipiens was found to be negative for Wolbachia.