September 11, 2023

Sample information
Picture
Photos by: Nathan N.
Location
Collection date 08/10/2023
Captive / Cultivated? Captive / Cultivated
Group Galileo Academy of Science and Technology
Observations

It had a proboscus, slender legs, wings
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex Culex pipiens

Methods
Extraction kit BABEC
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Did not produce 2nd green band of dna
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex pipiens was found to be negative for Wolbachia.
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