Ant, Camponotus pennsylvanicus

Sample information
Picture
Location
Collection date
Captive / Cultivated?
Group	Edmund Burke School
Observations	medium sized ant, was behind school running across pavement. Was about 80 degrees outside, sunny day. Last day of summer.
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	DNA Extraction, the lab went well with no struggles, protocol was followed, surfaces sterilized and pipette tips changed. PCR: there were 2 issues with the PCR, first the proportions of compounds in the cocktail were off, but we remade the cocktail so this did not affect the results and the Wolbachia PCR was botched at first but we redid it and got conclusive results Gel Electrophoresis, arthropod: got a clean bar for both Wolbachia and CO1 Arthropod gene. As for Wolbachia we at first had the issue of no bars, due to a PCR error, but we redid the PCR and Electrophoresis and got conclusive results
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	The bar was nice and solid. The sequencing had a low e value as well which shows that the gene was most likely wolbachia
Wolbachia 16S sequence	Download FASTA
Arthropod COI sequence	Download FASTA
Summary	The Camponotus pennsylvanicus was found to be postive for Wolbachia.

The Wolbachia Project Database