Periplaneta American

November 26, 2023

Sample information
Picture
Photos by: Matthew C.
Location
Collection date 09/18/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Small head, small abdomen, large thorax, 2 antenna, 2 wings, 4 legs, gray-ish black
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction: Although there were no mistakes in our extraction process it took a lengthy amount of time to crush down all of our arthropods as some were in big chunks

PCR: Every lane in our DNA ladder besides water was positive for the arthropod gene. Only 1 band was highlighted which was C#1 meaning that arthropod tested positive for the wolbachia gene. No mistakes were made during the PCR process and our controls worked well.

 

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Our confidence level is about halfway since we were very precise with every measurement and made sure to never cross contaminate. Although we didn’t make any mistakes the band infected with wolbachia was light meaning there is possibility our arthropod is not actually infected.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Blattodea was found to be postive for Wolbachia.
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