|Captive / Cultivated?
|Edmund Burke School
Small head, small abdomen, large thorax, 2 antenna, 2 wings, 4 legs, gray-ish black
|Arthropoda Hexapoda Insecta Blattodea
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
DNA extraction: Although there were no mistakes in our extraction process it took a lengthy amount of time to crush down all of our arthropods as some were in big chunks
PCR: Every lane in our DNA ladder besides water was positive for the arthropod gene. Only 1 band was highlighted which was C#1 meaning that arthropod tested positive for the wolbachia gene. No mistakes were made during the PCR process and our controls worked well.
|Explanation of confidence level
Our confidence level is about halfway since we were very precise with every measurement and made sure to never cross contaminate. Although we didn’t make any mistakes the band infected with wolbachia was light meaning there is possibility our arthropod is not actually infected.
|Wolbachia 16S sequence
|Arthropod COI sequence
| Download AB1
|The Blattodea was found to be postive for Wolbachia.