Black blow fly

December 15, 2023

Sample information
Picture
Photos by: Hailey F.
Location
Collection date 09/20/2023
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations

This fly was caught in a small terrestrial garden in the front of a high school in Maine. It was surrounded by mulch on one side and a concrete sidewalk on the other. It was caught on a 70 degree day in fall. This fly is 6 legged with veiny brownish -clear wings, a black body, a reddish brown head, and red eyes.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Left side: Arthropod                          Right side: Wolbachia

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I can’t be completely confident on the fact that I have positive Wolbachia because somehow during the protocol the H2O in the Wolbachia test got contaminated and it showed positive. This lowers my confidence on my bug having Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be postive for Wolbachia.
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