Smiley4: sarcophaga carnaria

October 24, 2024

Sample information
Picture
Photos by: Julia C., Svenia H.
Location
Collection date 08/18/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Caught alive on apple tree, frozen to death and stored in ethanol until DNA-extraction
Putative identification Arthropoda Hexapoda Insecta Diptera Sarcophagidae Sarcophaga Sarcophaga carnaria

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

ArthropodPCR-Gel2: During this PCR round arthropodic DNA was targeted. The relevant sample is located in lane 6.

WolbachiaPCR-Gel9: Here wolbachia was targeted. The relevant sample is located in lane 7.

The  results show that the sample was not infected with wolbachia

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There is no band which would indicate wolbachia presence. This can be trusted because other samples on the same gel tested positive for wolbachia. The control sample is clearly recognizable as well, which means the PCR-process was successful.

(Additionally, since the first round didn‘t generate usable results, the PCR was repeated by the biology teacher, which makes the results more credible.)
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Sarcophaga carnaria was found to be negative for Wolbachia.
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