GREAIF24AM – Hymenoptera

November 7, 2024

Sample information
Picture
Photos by: Athalea M.
Location No map location data available.
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Time: 2:31 PM
  • Habitat: Inside a tea olive bush on the side of the Science center
  • Method: Beating stick and net
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Two reasons why I’m so confident in my results is because of the A260/A280 ratio value, and the PCR lab. My insects DNA had a value of 2.28, which means that it is not contaminated with proteins or other bacteria. If it were less than 1.7, cross-contamination would occur, causing multiple bands to appear in my gel image. With PCR, I made sure that I micropipetted the right amount of solutions needed, and asked my instructor and partners questions whenever I was confused with a step, especially the most critical ones (ex. crushing the insects exoskeleton thoroughly in the tube). In addition, Hymenoptera in my local area has the lowest prevalence of Wolbachia (7.3%) out of all the orders. This made the chances of my insect specimen having Wolbachia very low.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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