FernLeaf LazyLizards Results (Stingbug)

February 12, 2025

Sample information
Picture
Photos by: JCK
Location
Collection date 12/11/2024
Captive / Cultivated? Wild-caught
Group FernLeaf Community Charter School
Observations

We found a stinkbug underneath Tanya Praters table in her classroom.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Pentatomidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

We first started with collecting our insects. we than extracted the DNA from the abdomen of the stinkbug, than went through the procedure of using our purified Dna prepared PCR samples separately for the arthropod gene and the wolbachia gene. for electrophoresis we used the mini PCR machines and ran our PCR products for 30 minutes each.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Well, within our gel electroscopes system we can see that our positive and negative were aligned correctly. the water contained no wolbachia, the negatives contained no wolbachia and the positives did. Our stinkbug showed positive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Pentatomidae was found to be postive for Wolbachia.
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