Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/04/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | FernLeaf Community Charter School |
| Observations | Triangular abdomen with mottled color, brown with white flecks. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Followed protocol, extracted DNA goop from abdomen of the sample of Halymorpha Halys. May have left exoskeleton in before the buffer AL was added. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Done as a school group, could have been contamination between samples. A negative result makes me think not, but it is always possible contamination occurred while inserting gels into the mini one.
|
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
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