NV 65 Differential Grasshopper

Sample information

Picture
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

The grasshopper was just hopping around with the other grasshoppers there and vibing in the grass.

Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods

Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Orthoptera was found to be negative for Wolbachia.
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