Cricket

May 4, 2021

Sample information
Picture
Location
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

I found the cricket next to a pine tree. There were a lot of crickets around. The cricket had 2 antennas, 6 legs, 2 palps, 13 sections, and 3 tails. It was about 70 degrees outside.
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The Gel for the wolbachia part did not have to correct wells. The pellet was a clear white with a gray tint.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Arthropod controls not sure about the bands in wolbachia positive  and arthropod negative band

wolbachia controls- not sure about negative control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
Report Inappropriate Post