Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | The millipede was found inside of a log. It is likely a detritivore. |
| Putative identification | Arthropoda Myriapoda Diplopoda Polydesmida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Our experiment failed, most likely in the DNA extraction step because we did not crush the arthropods enough. Both the Arthropod positive and the DNA positive results were successful, and they gave the expected results. The PCR was successful as well as the Gel electrophoresis, so the DNA extraction was the step that failed. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)