|Captive / Cultivated?||Wild-caught|
|Group||St. John High School USD 350|
The box elder bugs were found on a tree and seemed to be apart of a colony. The bugs estimated infection rate is 12.5%.
|Putative identification||Hexapoda Insecta Hemiptera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniPCR|
The boxelder bugs were stored in 90% ethanol for about six months prior to extraction.
DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.
PCR: Mini One Taq polymerase was used.
Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.
|Explanation of confidence level||
Lane 7 was double banded and the Wolbachia band matched up with the positive control.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Hemiptera was found to be postive for Wolbachia.|