Boxelder Bugs

February 11, 2021

Sample information
Picture
Location
Collection date 08/17/2020
Captive / Cultivated? Wild-caught
Group St. John High School USD 350
Observations

The box elder bugs were found on a tree and seemed to be apart of a colony. The bugs estimated infection rate is 12.5%.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The boxelder bugs were stored in 90% ethanol for about six months prior to extraction.

DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.

PCR: Mini One Taq polymerase was used.

Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Lane 7 was double banded and the Wolbachia band matched up with the positive control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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