|Captive / Cultivated?
|St. John High School USD 350
The box elder bugs were found on a tree and seemed to be apart of a colony. The bugs estimated infection rate is 12.5%.
|Arthropoda Hexapoda Insecta Hemiptera
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
The boxelder bugs were stored in 90% ethanol for about six months prior to extraction.
DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.
PCR: Mini One Taq polymerase was used.
Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.
|Explanation of confidence level
Lane 7 was double banded and the Wolbachia band matched up with the positive control.
|Wolbachia 16S sequence
|Arthropod COI sequence
|The Hemiptera was found to be postive for Wolbachia.