Pavement Ant

Sample information

Picture
Photos by: Ella C.
Location
Collection date 09/24/2020
Captive / Cultivated? Wild-caught
Group Hillsboro High School
Observations

This pavement ant was the second caught ant from my collection. It liked the sugar on my glove and was not frantic nor did it run away. It crawled around the glove until it was submerged in the ethanol. Had a thin black shell that covered the entirety of its body.

Putative identification Hexapoda Insecta

Methods

Extraction kit
DNA extraction location
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

I am highly confident that this insect did not contain Wolbachia. I know that the controls worked because the H2O control did not show any sort of smearing on the bands. This indicates to me that the process was done properly since H2O is not supposed to show any bands whatsoever. I know that this insect did not contain Wolbachia because the Wolbachia infected control showed a band on the gel electrophoresis, while the non-infected control showed nothing. My insect showed no bands for the Wolbachia specific primers, therefore I am highly confident that this insect is not Wolbachia positive.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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