Sample 2 – Mosquito

Sample information

Picture
Photos by: Kaitlynn C.
Location No map location data available.
Collection date 10/04/2020
Captive / Cultivated?
Group Toms River High School East
Observations

When I caught the second bug it was so much easier. The bug as long legs like the first but this mosquitoes body was even smaller which makes me believe there is no Wolbachia in it.

Putative identification Hexapoda Insecta Diptera Culicidae Culex

Methods

Extraction kit
DNA extraction location
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I think this bug does not have Wolbachia in it because its body was so much smaller than the first one. This makes me believe there is no Wolbachia in it.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
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