Unknown Black Fly

Sample information

Picture
Photos by: Isabella R.
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Bordenstein Lab
Observations

found on aster in a flowerbed, NE location in backyard behind suburban home

2mm long, all black, two wings, delicate legs

Putative identification Hexapoda Insecta Diptera

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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