NV 78 Mosquito

April 15, 2021

Sample information
Picture
Location No map location data available.
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Neuqua Valley High School
Observations

It was caught on a spider web it was stuck on outdoors during the fall at 55 degrees F in a terrestrial habitat.
Putative identification Arthropoda Myriapoda Chilopoda

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

In one of the gel images provided I found that there was no trace but in the other gel there was a light trace of band indicating it might be Wolbachia positive.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Chilopoda was found to be negative for Wolbachia.
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