Roly Poly

May 4, 2021

Sample information
Picture
Photos by: Matthew R
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Has antenna, 14 legs, no wings, legs have little hairs, it’s back is semi-clear, has a grey/brown exterior, brown underside, has a pair of eyes
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Partial abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet was a lightish brown but it was visible.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I felt like I did everything correctly but the dna extraction tripped me up a bit.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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