Flying Ant

April 27, 2022

Sample information
Picture
Location
Collection date 04/11/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The arthropod was found in a terrestrial environment near some small rocks. It was a wooded area near shrubs and flowers. Flying ants have elbowed antennas and long fore wings. It has a thin waist constricted at the thorax and small hind wings. The darkly-colored ant is slightly larger than a grain of rice.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Possible result mistakes:
insufficient amount of DNA, DNA extraction failure

Pellet Description: small, visible black segments of body churned up

 

 

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The positive control did not test for an arthropod, and the negative control tested to be an arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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