Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/06/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | |
| Putative identification | Arthropoda Myriapoda Chilopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The assigned confidence level for the observation is medium due to the detection of bands, albeit of faint intensity. This determination is rooted in the understanding that band formations typically signify underlying patterns or structures within the observed data. However, the diminished prominence of these bands raises certain uncertainties, thereby warranting a moderate confidence level. In order to enhance the academic tone, it is important to emphasize the empirical evidence of the observed bands while acknowledging the limitations imposed by their faint appearance, ultimately leading to the medium confidence rating. DNA concentration being less then optimal may have also led to above results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Chilopoda was found to be negative for Wolbachia. |
Water Boatman