|Captive / Cultivated?||Wild-caught|
Specimen collected from underneath a log. The specimen is an even dark brown and has two antennae, 7 pairs of legs, and two uropods; it possesses essential characteristics of the common woodlouse, commonly referred to as a sowbug.
|Extraction kit||DNeasy (Qiagen) blood and tissue kit|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||SYBR Safe|
10-fold dilutions (20µl→ 18µl of distilled water, 2µl of original sample) were performed on template PCR due to the original DNA concentrations of the sample being too high, causing no reading on the gel. The gel submitted is the gel taken of the diluted samples, the second gel overall.
|Explanation of confidence level||
The Wolbachia portion of the gel didn’t yield any results, including the controls(the only aspect visualized on the gel was the loading buffer). The only conclusive results were sample 2 in lane 2 of the arthropod portion of the gel, as a band is clearly pictured at around 700 bp(base pairs) on the gel. In addition, the arthropod controls yielded results at around 700 bp.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download FASTA
GCTGTTGGAACAGCCCTTAGGATAATTATTCGAACTGAGTTAGGAAACCCTGGGAGGTTAATTGGAGACGATCAGATTTATAATGTGATTGTTACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGCTTTGGTAATTGATTAGTACCTTTAATATTAGGGGCCCCTGATATAGCTTTTCCACGTATAAATAATATAAGATTTTGGCTTCTTCCCCCATCACTAATTTTGTTATTAAGAAGAGGTTTAGTTGAAAGAGGTGTTGGGACTGGCTGGACTGTCTACCCACCTTTGGCAAGTGGAATTGCCCATAGAGGGGCTTCAGTAGATTTAGGGATTTTTTCCCTTCATTTGGCTGGAGCTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACTGTAATTAATATACGAGCAGAGGGGATAAGGATAGATCGTGTCCCTCTATTTGTTTGGTCAGTGTTAATTACAGCAATTCTCTTGTTACTTTCACTACCAGTTTTATCAGGGGCCATTACTATGTTATTAACAGACCGTAATCTGAACACCTCTTTTTTCGACCCTAGGGGGGGAGGAGATCCTATCTTGTACCAACACTTATTTTGATTTTTTGGTCACACAAAABLAST at The Wolbachia Project BLAST at NCBI