Small ant

March 16, 2024

Sample information
Picture
Photos by: Sonia K
Location
Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I found this ant crawling in a group of ants on the sidewalk in the courtyard. I collected it in the afternoon, about 2pm, and it was a sunny summer day. I attached a photo of the ant colony from which I collected one ant. It is very small and black with brown legs.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Tetramorium

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My samples are in lanes 4 and 5.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

There were no bands matching arthropod or Wolbachia DNA in my samples. This indicates that some part of the testing process was performed incorrectly, as all tested arthropods should have been positive for arthropod DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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