Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 11/19/2020 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | St. John High School USD 350 |
| Observations | I purchased Triop eggs online and raised them. I was only able to get 3 to hatch out and those were the ones I extracted. |
| Putative identification | Arthropoda Crustacea Branchiopoda Notostraca |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The Triops were raised for about 2 weeks until they reached full size. They were then stored in 90% ethanol for a day until extraction. DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete. PCR: Mini One Taq polymerase was used. Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of the triops only had one bright band that did not match up with my two banded positive controls. All of the protocols were also followed exactly so I am confident they are negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Notostraca was found to be negative for Wolbachia. |
Water Boatman