|Captive / Cultivated?||Captive / Cultivated|
|Group||St. John High School USD 350|
I purchased Triop eggs online and raised them. I was only able to get 3 to hatch out and those were the ones I extracted.
|Putative identification||Crustacea Branchiopoda Notostraca|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniPCR|
The Triops were raised for about 2 weeks until they reached full size. They were then stored in 90% ethanol for a day until extraction.
DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.
PCR: Mini One Taq polymerase was used.
Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.
|Explanation of confidence level||
All of the triops only had one bright band that did not match up with my two banded positive controls. All of the protocols were also followed exactly so I am confident they are negative.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Notostraca was found to be negative for Wolbachia.|